Sorghum is the third most consumed cereal in Haiti. It is cultivated in almost all departments of Haiti. Studies conducted on the safety of sorghum food chain in Haiti are rare. This preliminary study is aimed at evaluating the aflatoxin (AF) contamination of products across three steps of sorghum food chain in South Haiti. Significantly different AF contaminations were noticed among samples (p<0.05). Mean AF concentrations are 5.40±0.62; 20.53±16.05 and 40.88±43.36 ppb in freshly harvested sorghum (FHS), sorghum street vendors (SSV) and manufactured sorghum products (MSP), respectively. Moreover, 50 and 44.44% of tested MSP and SSV had AF concentrations > 20 ppb (limit of US Food and Drugs Administration) while none of FHS is > 20 ppb. Findings suggest that MSP and SSV are the steps with the highest aflatoxin contamination in South Haiti. Complementary investigations are needed to confirm the present findings through more reliable AF quantification techniques.