Malaria remains a major public health problem in Myanmar. Anopheles larvae investigating study was conducted seasonally in Thayetchaung village, Bawbin dam areas, Bago division and Maybinther villages Myothit Township Magwe Division from January to December 2016. Larval survey was done in and around the all possible water bodies such as water pools, sand pools, water pockets, creeks, foot prints, ponds, Dam and pools along the sand banks of creeks and rivers were investigated within 3 kilo meters radius from surrounding areas of both villages. Year round data showed that a total of 513 and 311 habitats were investigated and found to be 96 (18.71%) and 51(16.39%) habitats were positive for Anopheles larvae in Bawbin and Maybinther respectively. 87.63 and 92.31% of the larvae collected from survived were emerged to adults. Eight breeding habitats from Bawbin and 5 breeding habitats were observed from Maybinther village respectively. After identification, An. maculatus, An. annularis, An, vagus, An. culicifacies, An. minimus and An. stephensi larvae were observed in both areas but only An. philippnansis larvae was collected in water pools, rice field and dam in Bawbin area although An. philippnansis and An. babirostris larvae were lacked in Maybinther village in all season. The highest numbers of Anopheles larvae were collected in sand pools followed by water pools in cold season. Highest number of An. vagus larvae was collected in ponds and foot prints followed by An. maculatus in water pools and water pockets in raining season. An. culicifacies was found in high density in sand pools of creek in cold season in both areas. High number of An. annularis was present in water pools in cold season in Maybinther village. Low number of An. annularis, An. vagus and An. culicifacies larvae were observed in dry season in both areas. The main vector of malaria such as An. minimus was observed in rice field and send pool in Bawbin and water pool and sand pool of creek in Maybinther villages in raining and cold season. Density of collected An. culicifacies larvae was much higher in Maybinther, 2.9 fold higher than Bawbin village. This study concluded that water pools, water pockets and sand pools creeks might play a significant role in rural areas to breed high number of Anopheles mosquitoes in both study areas followed by foot prints, canals, dam, rice fields and ponds. It is hoped that the information about the distribution of larvae in the breeding habitats was gathered from this study will help to broaden the understanding with regards to the geography, biology and ecology of mosquito breeding sites, and thus effective and efficient larval control measures can be applied.